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Bacteria associated with Amblyomma cajennense tick eggs Genet. Mol. Biol.
Machado-Ferreira,Erik; Vizzoni,Vinicius Figueiredo; Piesman,Joseph; Gazeta,Gilberto Salles; Soares,Carlos Augusto Gomes.
Abstract Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amblyomma cajennense; Tick eggs bacteria; Staphylococcus; Ixodidae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400477
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Uso da condutividade elétrica do leite para detecção de mastite subclínica caprina. Infoteca-e
SOUZA, V. de; LIMA, A. R.; MOURA, J. W. F.; ANGELO, F. F.; ALCINDO, J. F.; MESQUITA, F. L. T. de.
O trabalho avalia a utilização do teste da condutividade elétrica como ferramenta para o diagnóstico da mastite em amostras de leite obtidas em diferentes rebanhos de caprinos leiteiros.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Caprino leiteiro; Teste de Mamite; Doença Animal; Bactéria; Glândula Mamaria; Goat diseases; Brazil.; Mastitis; Bacterial infections; Disease diagnosis; Staphylococcus; Mammary gland diseases.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1116655
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Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae ArchiMer
Sanchez-rodriguez, Diana Elizabeth; Ortiz-aguirre, Ismael; Aguila-ramirez, Ruth Noemi; Guadalupe Rico-virgen, Erika; Gonzalez-acosta, Barbara; Hellio, Claire.
One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The...
Tipo: Text Palavras-chave: Epibionts; Extracts; Shewanella; Staphylococcus; Biofouling; Antifouling.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00616/72762/72271.pdf
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Identification of 129 Micrococcaceae strains isolated from food of animal origin ArchiMer
Delarras, C; Guichaoua, C.; Caprais, Marie-paule.
129 strains of Micrococcaceae were isolated from food of animal origin (minced meat, cakes with confectioner's custard) on Baird-Parker medium. 120 were Staphylococcus and 9 Micrococcus, according to the ID 32 STAPH-System (1989). The results were analyzed using a computer program. 60% of these 120 Staphylococcus strains of animal origin were coagulase positive S. aureus (aurease+, Rapidec Staph technique). 46% were resistant to novobiocin (5 microg/ml), 13 to 44% were identified with negative discordant tests using this micromethod. 44% produced no acetoin, 35% had no urease and 15% no arginine dihydrolase. 48 Staphylococcus strains (40%) were identified as human coagulase negative Staphylococcus species (strains: 39; species: 9) or as animal species...
Tipo: Text Palavras-chave: Staphylococcus; Nitrofurantoin; Aurease; ID 32 STAPH; Numerical identification.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00071/18273/15838.pdf
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Prevalencia y perfil de resistencia a betalactámicos en estafilococos de perros y gatos InVet
Denamiel,G; Puigdevall,T; Más,J; Albarellos,G; Gentilini,E.
El objetivo de este trabajo fue conocer la prevalencia de especies y perfil de resistencia a betalactámicos en estafilococos aislados de infecciones en caninos y felinos. En perros, de 207 aislamientos, el porcentaje de las especies identificadas fue: S. intermedius 29,5%, S. aureus y S. simulans 20,8%, S. saprophyticus 12,6%, S. chromogenes 10,6%, S. haemolyticus 3,9% y S. epidermidis 1,9%. De 63 aislamientos en gatos: S. simulans 33,3%, S. intermedius y S. aureus 15,4%, S. saprophyticus 11,1%, S. haemolyticus 6,34%, S. chromogenes y S. epidermidis 3,5%. En perros, el porcentaje de resistencia a penicilina fue: S. intermedius 47,5%, S. aureus 34,8% y S. saprophyticus 7,7%. Los aislamientos que expresaron meticilina resistencia correspondieron: S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Betalactámico; Resistencia; Staphylococcus; Perro; Gato.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982009000200006
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Identification and antimicrobial susceptibility of Staphylococcus from home-treated peritoneal dialysis patients J. Venom. Anim. Toxins incl. Trop. Dis.
Batalha,JEN; Cunha,MLRS; Montelli,AC; Barreti,P; Caramori,JCT.
Staphylococcus aureus is the main agent of infections during peritoneal dialysis (PD). The presence of S. aureus in the nasal cavity has been extensively studied and suggested as a risk factor of dialysis-related infections, whereas coagulase-negative Staphylococcus (CNS) species are frequently considered part of the normal human microbiota. The aim of this study was to identify Staphylococcus in the nasal cavity, pericatheter skin and peritoneal effluent from PD patients, as well as to evaluate the antimicrobial activity evolution in vitro. Thirty-two chronic PD patients were observed during 12 months and had nasal and pericatheter skin samples collected for culture. When peritonitis was detected, samples were also collected from the peritoneal effluent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peritoneal dialysis (PD); Peritonitis; Risk factor; Staphylococcus; Coagulase-negative Staphylococcus (CNS).
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200005
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Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. J. Venom. Anim. Toxins incl. Trop. Dis.
Marconi,C.; Cunha,M. L. R. S.; Araújo Jr,J. P.; Rugolo,L. M. S. S..
Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Delta toxin; PCR; Staphylococcus; Coagulase-negative staphylococci.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200004
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Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina PAB
Loguercio,Andrea Pinto; Groff,Ana Cláudia Mello; Pedrozzo,Andréia Folleto; Witt,Niura Mazzini; Silva,Mariana Sá e; Vargas,Agueda Castagna de.
Este trabalho foi desenvolvido com o objetivo de avaliar a atividade in vitro do extrato alcoólico de própolis, contra agentes da mastite bovina, comparando-o aos principais antimicrobianos utilizados no tratamento convencional. Foram utilizados 36 isolados coagulase-positivos de Staphylococcus sp. e 27 isolados de Streptococcus sp.; 94,4% dos Staphylococcus sp. e 85,2% dos Streptococcus sp. foram susceptíveis ao extrato de própolis.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Staphylococcus; Streptococcus; Susceptibilidade; Antimicrobianos.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000200021
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Effect of chitosan coating on contamination of fresh bovine meat sold in the open market Rev. Ciênc. Agron.
Silva,Alessandra Santana; Sampaio,Adriana Pereira; Santos,Marly Silveira; Souza,Bartolomeu Warlene Silva de; Evangelista-Barreto,Norma Suely.
ABSTRACT This study aimed to evaluate the sanitary status of fresh bovine meat sold at the open market in Jiquiriça municipality, Brazil and validate the use of chitosan as an antimicrobial coating to control the bioburden. In total, we evaluated 30 samples of fresh beef for the presence of coagulase-positive staphylococci and Salmonella spp. Furthermore, stores selling fresh meat products were evaluated for compliance with sanitary regulations set by the Ministry of Health in Brazil. To evaluate the antimicrobial activity of chitosan two sets of meat samples were tested, the test group consisted of contaminated meat coated with 2% chitosan solution whereas the control group consisted of untreated contaminated meat. It was found that fresh bovine meat at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Staphylococcus; Internal meat temperature; Antimicrobial activity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100038
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Presença de Staphylococcus spp. produtores de enterotoxinas e da toxina da síndrome do choque tóxico em manipuladores de queijo de cabra Arq. Bras. Med. Vet. Zootec.
Rapini,L.S.; Cerqueira,M.M.O.P.; Carmo,L.S.; Veras,J.F.; Souza,M.R..
Agruparam-se 167 cepas de Staphylococcus, isoladas de diferentes sítios (fossas nasais, orofaringe, palma das mãos e leitos subungueais), de dois manipuladores de queijos de leite de cabra, em pools (45), de acordo com a similaridade da espécie e o local de origem, e testou-se o seu potencial de produção de enterotoxinas (SE) A, B, C e D, e da toxina da síndrome do choque tóxico (TSST-1). Constatou-se que 62,2% dos pools foram capazes de produzir, de forma individual ou associada, SEA (33,3%); SEB (46,7%); SEC (8,9%); SED (4,4%) e TSST-1 (4,4%). Dos pools enterotoxigênicos, 96,4% corresponderam a espécies coagulase negativa (Staphylococcus epidermidis e Staphylococcus cohnii). A capacidade de produção de SE e de TSST-1 pelas cepas de Staphylococcus spp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cabra; Manipulador; Queijo; Staphylococcus; Enterotoxinas; TSST-1.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600019
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Mastite subclínica por Staphylococcus coagulase negativa em ovinos de corte Arq. Bras. Med. Vet. Zootec.
Tejada,T.S.; Silva,D.T.; Dias,P.A.; Conceição,R.C.S.; Müller Neto,H.; Timm,C.D..
Microorganisms that cause mastitis were isolated in a herd of sheep and their sensitivity to antimicrobials was tested. Twenty-one sheep had their mammary glands monitored during lactation. Eighty-eight milk sample from CMT-positive sheep were analyzed in order to isolate the etiologic agent of mastitis. Coagulase negative Staphylococcus were isolated from 29.5% of samples. Ampicillin and penicillin were the antimicrobials with higher number of resistant strains. The importance of coagulase negative staphylococci has been demonstrated, especially S. lentus and S. sciuri as a cause of ovine mastitis.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Coagulase negativa; Mastite; Ovinos; Staphylococcus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400039
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Contagem de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas e toxina da síndrome do choque tóxico em amostras de leite cru refrigerado Arq. Bras. Med. Vet. Zootec.
Lamaita,H.C.; Cerqueira,M.M.O.P.; Carmo,L.S.; Santos,D.A.; Penna,C.F.A.M.; Souza,M.R..
Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores de propriedades rurais do estado de Minas Gerais quanto à contagem e identificação de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas (SE) e da toxina da síndrome do choque tóxico (TSST-1). Staphylococcus sp. foi detectado em 100% das amostras de leite de tanque refrigerador em contagens que variaram de 1,0 × 10(5) a 2,5 × 10(7) UFC/ml (média = 5,60 log UFC/ml; s = 0,53 e CV = 9,5%). Isolaram-se e identificaram-se 436 estirpes como: S. aureus, S hyicus, S. epidermidis, S. intermedius, S. cohnii, S. sciuri, S. schleirferi e S. delphini. As estirpes de mesmo perfil bioquímico, oriundas da mesma amostra, foram agrupadas (pools) e induzidas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leite cru; Staphylococcus; Enterotoxina; TSST-1.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500017
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Perfil de resistência antimicrobiana de cepas de Staphylococcus sp. isoladas de queijo tipo coalho Arq. Bras. Med. Vet. Zootec.
Rapini,L.S.; Teixeira,J.P.; Martins,N. E.; Cerqueira,M.M.O.P.; Souza,M.R.; Penna,C.F.A.M..
The antimicrobial resistance profile of 45 Staphylococcus strains isolated from 10 samples of Brazilian type "coalho" cheese was evaluated against eight antibiotics used either in human or in veterinarian medicine. The tested antibiotics decreasing resistance degree was: penicillin (100.0%), tetracycline (91.0%), vancomycin (75.5%), gentamicin (71.1%), oxacillin (66.7%), erythromycin (60.0%), cephalothin (48.9%) and sulphazothrin (26.7%). The high frequency of Staphylococcus strains presenting resistance to the tested antibiotics, emphasizes the importance of the control of the abusive use of antibiotics by medical and veterinarian subjects.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Staphylococcus; Antibiograma; Queijo tipo coalho.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000100022
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Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification Arq. Bras. Med. Vet. Zootec.
Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M..
ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669
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The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin Arq. Bras. Med. Vet. Zootec.
Dias,R.S.; Carmo,L.S.; Heneine,L.G.D.; Rocha,P.H.; Barbosa,C.F.; Rodrigues,R.J.; Linardi,V.R..
Acute toxicity test (LD-50) using toxic shock syndrome toxin (TSST-1) was tested in BALB/c, C57BL/6 and Swiss mice. Animals (n = 10) were intraperitoneally injected with TSST-1 (0.01-10.0µg/mouse) followed 4h later by potentiating dose of lipopolysaccharide (75.0µg of LPS - E. coli O111:B4) and cumulative mortality was recorded over 72h. Control animals received either TSST-1 or LPS alone. The data were submitted to qui-Square test and acute toxicity test was calculated by probit analysis (confidence limits expressed as µg toxin/kg). BALB/c mice was the most sensitive (20.0µg/kg, 95% confidence limits: 9.0-92.0) followed by C57BL/6 (38.5µg/kg, 95% confidence limits: 9.11- 401.6). Data from Swiss mice was not conclusive, indicating only low sensitivity....
Tipo: Info:eu-repo/semantics/report Palavras-chave: Mice; TSST-1; Acute toxicity (LD-50); Staphylococcus.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100024
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Identification of Staphylococcus spp. isolated during the ripening process of a traditional minas cheese Arq. Bras. Med. Vet. Zootec.
Borelli,B.M.; Lacerda,I.C.A.; Brandão,L.R.; Vianna,C.R.; Ferreira,M.C.; Gomes,F.C.O.; Carmo,L.S.; Heneine,L.G.D.; Rosa,C.A..
The population dynamics of Staphylococcus spp. was studied during the ripening of Canastra Minas cheese at three farms located in the State of Minas Gerais, Brazil. The presence of coagulase (coa), thermonuclease (nuc), and enterotoxin (sea, seb, sec, and sed) genes was investigated in Staphylococcus strains isolated during the 60-day cheese-ripening period. The presence of the staphylococcal enterotoxins A, C, and D was also investigated in the cheese samples. Cheese samples that were matured for 0, 7, 15, 30, and 45 days presented staphylococci counts from 10³ to 10(8)cfu/g. All isolates considered coagulase-positive by physiological tests had the coa gene. However, no association was observed between the results obtained with biochemical tests and those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canastra Minas cheese; Staphylococcus; Enterotoxin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200028
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Anti-staphylococcal effectiveness of nisaplin in refrigerated pizza doughs BABT
Freitas,Wilma Carla de; Souza,Evandro Leite de; Sousa,Cristina Paiva de; Travassos,Antônio Eustáquio Resende.
This study evaluated the effectiveness of nisaplin, commercial product having nisin as active component, in decreasing the staphylococcal population in refrigerated pizza doughs. The refrigerated pizza dough pieces randomly chosen were dipped in the solutions with nisaplin concentrations of 1.0 x 10-3 g and 1.0 x 10-2 g nisaplin/mL named for the treatment A and B and kept under refrigeration (7 °C ± 1 °C). On times 0, 15 and 30 days post treatment the Staphylococcus spp. count was carried out. The results showed that both nisaplin treatments were able to reduce the Staphylococcus spp. count (CFU/g) in the refrigerated pizza doughs. However, only treatment B showed statistically significant reducer effect (p < 0.05) on the count providing a decrease of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisaplin; Nisin; Dough; Staphylococcus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300021
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Methicillin-resistant Staphylococcus lugdunensis carrying SCCmec type V misidentified as MRSA BJID
Pereira,Eliezer Menezes; Schuenck,Ricardo Pinto; Nouér,Simone Aranha; Santos,Kátia Regina Netto dos.
Staphylococcus lugdunensis is a rare cause of severe infections and clinical manifestations are similar to those related to S. aureus infection. We describe a hospital-acquired bacteremia due to methicillin-resistant Staphylococcus lugdunensis, misidentified as methicillin-resistant S. aureus. The oxacillin MIC was 16 µg/mL and the mecA gene and SCCmec type V were determined by PCR. Although treatment had been appropriated, the patient died after rapid progressive respiratory failure and another nosocomial sepsis. It is important not only to identify S. lugdunensis in view of its clinical course, but also to determine its susceptibility to oxacillin by detecting the mecA gene or its product.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Staphylococcus; Methicillin resistance; Bacteremia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300020
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Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil BJID
Pereira,Jussyêgles Niedja da Paz; Rabelo,Marcelle Aquino; Lima,Jailton Lobo da Costa; Neto,Armando Monteiro Bezerra; Lopes,Ana Catarina de Souza; Maciel,Maria Amélia Vieira.
Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Clindamycin; Inducible resistance; Erm genes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300010
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Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates BJID
Coutinho,Vivian de Lima Spode; Paiva,Rodrigo Minuto; Reiter,Keli Cristine; de-Paris,Fernanda; Barth,Afonso Luis; Machado,Alice Beatriz Mombach Pinheiro.
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Resistance; Erm genes; Macrolides.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004
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